MIR146A inhibits JMJD3 expression and osteogenic differentiation in human mesenchymal stem cells
摘要：Abstract Chromatin remodeling is important for cell differentiation. Histone methyltransferase EZH2 and histone demethylase JMJD3 (KDM6B) modulate levels of histone H3 lysine 27 trimethylation (H3K27me3). Interplay between the two modulators influence lineage specification in stem cells. Here, we identified microRNA MIR146A to be a negative regulator of JMJD3. In the osteogenic differentiation of human mesenchymal stem cells (hMSCs), we observed an upregulation of JMJD3 and a downregulation of MIR146A. Blocking JMJD3 activity in differentiating hMSCs reduced transcript levels of osteogenic gene RUNX2. H3K27me3 levels decreased at the RUNX2 promoter during cell differentiation. Modulation of MIR146A levels in hMSCs altered JMJD3 and RUNX2 expression and affected osteogenic differentiation. We conclude that JMJD3 promotes osteogenesis in differentiating hMSCs, with MIR146A regulating JMJD3.
关键词：EZH2 enhancer of zeste homolog 2; enhancer of zeste homolog 2; H3K27me3 histone H3 lysine 27 trimethylation; histone H3 lysine 27 trimethylation; hMSCs human mesenchymal stem cells; human mesenchymal stem cells; JMJD3 jumonji domain containing 3; jumonji domain containing 3; PRC2 Polycomb Repressive Complex 2; Polycomb Repressive Complex 2; RUNX2 runt related transcription factor 2; runt related transcription factor 2; 3′ UTR 3′ untranslated region; 3′ untranslated region; Stem cells; MicroRNA; Jumonji domain containing 3; MIR146A; Osteogenesis; Cell differentiation
论文方向：[{"id":13,"name":"细胞生物学"},{"id":879,"name":"生物化学"}]
发表期刊：FEBS Letters Volume 588, Issue 9
发表时间：Fri May 02 00:00:00 CST 2014
数字识别码：10.1016/j.febslet.2014.03.057
是否作者本人： 否
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