RNA interference (RNAi) induction with various types of synthetic oligonucleotide duplexes in cultured human cells
作者:Hirohiko Hohjoh
摘要:Abstract Various types of synthetic oligonucleotide duplexes against the Photinus luciferase gene were tested on their induction of the sequence-specific RNA interference (RNAi) activity in transfected human cells. Results indicate that RNA duplexes with ribonucleotide 3′ overhangs rather than those with deoxyribonucleotide 3′ overhangs induce more efficient RNAi activity, and that sense-stranded DNA/antisense-stranded RNA hybrids induce a moderate RNAi activity. These results suggest that there is a difference in the potential of oligonucleotide duplexes to be RNAi mediators, i.e. short interfering RNAs (siRNAs), between human RNAi and invertebrate RNAi. The data further show that different siRNAs induce different levels of RNAi.
关键词:RNA interference; Synthetic oligonucleotide duplex; Cultured human cell; RNAi, RNA interference; siRNA, short interfering RNA; dsRNA, double-stranded RNA; PKR, interferon-inducible, dsRNA-activated protein kinase; nt, nucleotide
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发表期刊:FEBS Letters Volume 521, Issues 1–3
发表时间:Wed Jun 19 00:00:00 CST 2002
数字识别码:10.1016/S0014-5793(02)02860-0
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