ADP-evoked phospholipase C stimulation and adenylyl cyclase inhibition in glioma C6 cells occur through two distinct nucleotide receptors, P2Y1 and P2Y12
作者:, Jolanta Barańska
摘要:Abstract In this study we characterized the subtypes of nucleotide P2Y receptors that respond to ADP in glioma C6 cells. Direct visualization of phosphatidylinositol 4,5-bisphosphate at the cell surface revealed that extracellular ADP activates phospholipase C (PLC). Knock-down of P2Y1 receptor with antisense oligonucleotide, as well as treatment with MRS2179 and pyridoxal-phosphate-6-azophenyl-2′,4′-disulfonic acid (P2Y1 antagonists), attenuates receptor-mediated PLC activity. Adenylyl cyclase inhibition by ADP remains unchanged under these conditions. Reverse transcription-PCR analysis showed that P2Y12 receptor is expressed in C6 cells. We therefore conclude that, in glioma C6 cells, two P2Y receptor subtypes are present: P2Y1, coupled to PLC, and P2Y12, negatively coupled to adenylyl cyclase.
关键词:Nucleotide receptor; P2Y1; P2Y12; Phospholipase C; Adenylyl cyclase; Glioma C6 cell; [Ca2+]i, intracellular Ca2+ concentration; GFP, green fluorescent protein; InsP3, inositol 1,4,5-trisphosphate; MEM, minimum essential medium; PIP2, phosphatidylinositol 4,5-bisphosphate; PLC, phospholipase C; PPADS, pyridoxal-phosphate-6-azophenyl-2′,4′-disulfonic acid; PTX, pertussis toxin
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发表期刊:FEBS Letters Volume 513, Issues 2–3
发表时间:Wed Feb 27 00:00:00 CST 2002
数字识别码:10.1016/S0014-5793(02)02255-X
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