Phorbol ester-induced differentiation of L6 myogenic cells involves phospholipase D activation
摘要：Abstract TPA, a potent PKC activator, inhibits myogenic differentiation and activates phospholipase D (PLD). We evaluated the involvement of PLD in the TPA effects on L6 myoblasts differentiation. TPA, at concentrations inhibiting differentiation of L6 cells, induced a strong, though transient, PLD activation. Surprisingly, at nanomolar concentration, TPA induced both myogenic differentiation and sustained activation of PLD. Differential effect of TPA can be ascribed to PKC downregulation induced by highest TPA concentrations. TPA-induced differentiation was inhibited by 1-butanol, confirming the involvement of PLD in this effect. These data suggest that prolonged elevation of PLD activity is required for myogenic differentiation.
关键词：TPA, 12-O-Tetradecanoylphorbol 13-acetate; AVP, Arg8-vasopressin; PLD, phospholipase D; PKC, protein kinase C; Phospholipase D; Phosphatidic acid; Muscle; Myogenic differentiation; Protein kinase C; Phorbol esters
论文方向：[{"id":13,"name":"细胞生物学"},{"id":879,"name":"生物化学"}]
发表期刊：FEBS Letters Volume 577, Issue 3
发表时间：Fri Nov 19 00:00:00 CST 2004
数字识别码：10.1016/j.febslet.2004.10.036
是否作者本人： 否
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