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2024-03-24 15:43:57
马来酰亚胺与蛋白质中巯基反应问题我用到一种偶联剂，用偶联剂中的马来酰亚胺与蛋白质中的巯基反应，但是偶联剂的使用说明上说“molecules to be reacted with the maleimide moiety must have free(reduced) sulfhydryls.For proteins,reduce disulfide bonds using 5mM TCPE(1:100 dilution of bond-breaker TCEP solution) for 30minutes at room temperature, followed by two passes through a suitable desalting column” |
