Downregulation of CCND1 and CDK6 by miR-34a induces cell cycle arrest
作者:, Xiaofei Zheng
摘要:miRNAs regulate gene expression by inhibiting translation or by targeting messenger RNA (mRNA) for degradation in a post-transcriptional fashion. In the present study, we show that ectopic expression of miR-34a reduces both mRNA and protein levels of cyclin D1 (CCND1) and cyclin-dependent kinase 6 (CDK6). We also demonstrate that miR-34a targets the 3′-untranslated mRNA region of CCND1 as well as CDK6, which in turn interferes with phosphorylation of retinoblastoma. In addition, we show that overexpression of miR-34a induces a significant G1 cell-cycle arrest in the A549 cell line. Taken together, our data suggest that the effects of miR-34a on G1 cell cycle arrest are through the down-regulation of CCND1 and CDK6, which is associated with other targets of miR-34a either additively or synergistically.
关键词:miRNA microRNA; microRNA; 3′-UTR 3′-untranslated region; 3′-untranslated region; mRNA messenger RNA; messenger RNA; CCND1 cyclin D1; cyclin D1; CCND3 cyclin D3; cyclin D3; CCNE2 cyclin E2; cyclin E2; CDK4/6 cyclin-dependent kinase 4/6; cyclin-dependent kinase 4/6; MET hepatocyte growth factor receptor; hepatocyte growth factor receptor; MicroRNA; miR-34a; CCND1; CDK6; Cell cycle
论文方向:[{"id":13,"name":"细胞生物学"},{"id":879,"name":"生物化学"}]
发表期刊:FEBS Letters Volume 582, Issue 10
发表时间:Wed Apr 30 00:00:00 CST 2008
数字识别码:10.1016/j.febslet.2008.03.057
是否作者本人: 否
版权及免责声明:
本网站所有论文文件均系用户自行上传或提供,本网站对其内容准确性及合法性概不负责,亦不承担任何法律责任。论文版权归原作者及原出处所有。
如您发现网站其他用户上传的论文有侵犯您的姓名权、隐私权、著作权或其他合法权益现象的,请及时与本网站联系并附加相关权利证明文件,以便本网站及时作出处理,维护您的合法权益。
本网站拥有对此声明的最终解释权。
{replyUser1} 回复 {replyUser2}:{content}