Lymphotoxin α, a novel target of posttranscriptional gene regulation by HuR in HepG2 cells
摘要：Abstract The role of the RNA-binding protein human antigen R (HuR) in hepatocarcinogenesis is still elusive. By employing short hairpin (sh)RNA-dependent knockdown approach, we demonstrate that lymphotoxin α (LTα) is a target of posttranscriptional gene regulation by HuR in hepatocellular carcinoma (HepG2) cells. Consequently, the increased mRNA decay upon HuR depletion significantly affects lymphotoxin expression at both, the mRNA and protein level. Biotin-pulldown assay showed that HuR specifically interacts with the 3′-untranslated region (3′-UTR) of the LTα mRNA. Furthermore, electrophoretic mobility shift assay (EMSA) implicates that the RNA-binding critically depends on the RNA recognition motif 2 (RRM2) and the hinge region of HuR.
关键词：ARE AU-rich element; AU-rich element; EMSA electrophoretic mobility shift assay; electrophoretic mobility shift assay; HCC hepatocellular carcinoma; hepatocellular carcinoma; HuR human antigen R; human antigen R; LTα lymphotoxin α; lymphotoxin α; shRNA short hairpin RNA; short hairpin RNA; 3′-UTR 3′-untranslated region; 3′-untranslated region; RRM RNA recognition motif; RNA recognition motif; AU-rich element; Hepatocellular carcinoma; Lymphotoxin α; mRNA-stability
论文方向：[{"id":13,"name":"细胞生物学"},{"id":879,"name":"生物化学"}]
发表期刊：FEBS Letters Volume 589, Issue 15
发表时间：Wed Jul 08 00:00:00 CST 2015
数字识别码：10.1016/j.febslet.2015.05.015
是否作者本人： 否
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