Mesenchymal stem cells attenuate cardiac fibroblast proliferation and collagen synthesis through paracrine actions
摘要：Abstract Mesenchymal stem cells (MSC) transplantation has been shown to decrease fibrosis in the heart; however, whether MSC directly influence the function of cardiac fibroblasts (CFB) remains unknown. MSC-conditioned medium significantly attenuated proliferation of CFB compared with CFB-conditioned medium. MSC-conditioned medium upregulated antiproliferation-related genes such as elastin, myocardin and DNA-damage inducible transcript 3, whereas CFB-conditioned medium upregulated proliferation-related genes such as alpha-2-macrogrobulin and v-kit Hardy–Zuckerman 4 feline sarcoma viral oncogene homolog. MSC-conditioned medium significantly downregulated type I and III collagen expression, and significantly suppressed type III collagen promoter activity. MSC may exert paracrine anti-fibrotic effects at least in part through regulation of CFB proliferation and collagen synthesis.
关键词：MSC mesenchymal stem cell; mesenchymal stem cell; CFB cardiac fibroblast; cardiac fibroblast; MMP matrix metalloproteinase; matrix metalloproteinase; qRT-PCR quantitative real-time reverse-transcription polymerase chain reaction; quantitative real-time reverse-transcription polymerase chain reaction; Gapdh glyceraldehyde 3-phosphate dehydrogenase; glyceraldehyde 3-phosphate dehydrogenase; A2m alpha-2-macroglobulin; alpha-2-macroglobulin; Kit v-kit Hardy–Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy–Zuckerman 4 feline sarcoma viral oncogene homolog; Catna1 catenin alpha 1; catenin alpha 1; Rarb retinoic acid receptor beta; retinoic acid receptor beta; Eln elastin; elastin; Myocd myocardin; myocardin; Ddit3 DNA-damage inducible transcript 3; DNA-damage inducible transcript 3; Mesenchymal stem cell; Cardiac fibroblast; Cell proliferation; Collagen; Paracrine effect; Collagenase activity
论文方向：[{"id":13,"name":"细胞生物学"},{"id":879,"name":"生物化学"}]
发表期刊：FEBS Letters Volume 581, Issue 21
发表时间：Tue Aug 21 00:00:00 CST 2007
数字识别码：10.1016/j.febslet.2007.07.028
是否作者本人： 否
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